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The binding of heparin to cTnI may well decrease immunoreactivity, dependant upon the heparin concentration in sample assortment tubes (e.g., heparin at a focus of 90 U/mL is noted to result in a lessen of roughly twenty% in cTnI focus).[33] The influence of heparin on cTnI immunoassays might be induced by modifications within the sample matrix.Th
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The principle on the cTnT measurement is definitely an ELISA one particular-action sandwich assay using streptavidin know-how and electrochemiluminescence detection.[fifty four] In the main incubation move (immunological reaction), the cTnT in the sample reacts using a biotinylated mouse monoclonal anti-cTnT antibody and also a monoclonal cTnT-prec
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